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Fig. 8 | BMC Plant Biology

Fig. 8

From: AIP1 is a novel Agenet/Tudor domain protein from Arabidopsis that interacts with regulators of DNA replication, transcription and chromatin remodeling

Fig. 8

AIP1 protein interactions. a Yeast two hybrid assay with the C-terminal of AIP1 (aa 540-723) fused with GAL4 DAD (AIP1-C-Term AD;) against ARIA, AIP1 and LHP1 full-length CDSs fused with GAL4 DBD. GAL4 DBD empty vector was used as negative control. Details of the constructs can be found in Additional file 13. Yeast transformation was selected in -L-T (SD medium lacking Leucine and Tryptophan), and protein interactions were selected in -L-T-H or -L-T-H-A (SD medium lacking Leucine, Tryptophan and Histidine, or Histidine and Adenine. b GST pulldown of bacterially expressed recombinant GST-AIP1 with HIS-AIP1, or HIS-ARIA, or HIS-LHP1. HIS-tag interacting proteins were assayed with antibodies anti-HIS in immunoblots. c Semi-in vivo pulldown assay of bacterially expressed recombinant GST-AIP1 and protein lysates of Arabidopsis 10-day-old plants. Histone interacting proteins were assayed with Anti-H1, Anti H2B, Anti-H3 and Anti-H4 antibodies in immunoblots

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