Fig. 1From: One amino acid makes the difference: the formation of ent-kaurene and 16α-hydroxy-ent-kaurane by diterpene synthases in poplarPhylogenetic tree of putative kaurene synthase-(like) enzymes (KS(L)) and copalyl diphosphate synthases (CPS). The phylogenetic relationship of putative KS(L) and CPS synthases from P. trichocarpa to KS(L) and CPS from other plant species is shown. The tree was inferred with the neighbor-joining method and n = 1000 replicates for bootstrapping. Bootstrap values are shown next to each node. TPS-c and TPS-e, represent established TPS subfamilies [13]. PtTPS1 was used as an outgroup. KS: ent-kaur-16-ene synthase, SMS: stemar-13-ene synthase, LPPS: 8-hydroxy-copalyl diphosphate synthase, CPS: copalyl diphosphate synthase, Nt: Nicotiana tabacum, Cm: Cucurbita maxima, At: Arabidosis thaliana, Os: Oryza sativa, Pg: Picea glauca, Potri: Populus trichocarpa, Sm: Salvia miltiorrhiza, Pp: Physcomitrella patens Back to article page