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Fig. 6 | BMC Plant Biology

Fig. 6

From: The strigolactone biosynthesis gene DWARF27 is co-opted in rhizobium symbiosis

Fig. 6

Expression of MtD27, MtCCD7 and MtCCD8 upon treatment with rhizobium LCOs. a Relative transcript abundance as determined by qRT-PCR of MtD27, MtCCD7 and MtCCD8 in M. truncatula root susceptible zones 3 h after mock or rhizobium LCO (10-9 M) treatment. RNA was isolated from plants grown in Fåhraeus slides. b Relative transcript abundance as determined by qRT-PCR of MtD27, MtCCD7 and MtCCD8 in M. truncatula root susceptible zones 3 or 6 h after mock or rhizobium LCO (10-9 M) treatment. RNA was isolated from plants grown on agar-solidified Fåhraeus medium supplemented with 1 μM AVG. Data shown represent means of 2-3 biological replicates that each were analyzed in 3-fold (technical replicates) ± SEM. For each gene, transcript abundance was normalized against that of the mock-treated wild type. Different letters above bars indicate statistical difference (p < 0.05, students’ t-test)

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