Fig. 2From: Genome-wide survey and expression analysis of the OSCA gene family in riceMultiple sequence alignment and transmembrane region of the DUF221 conserved region in OsOSCAs. Multiple sequence alignments of OSCAs were performed using DNAman and the transmembrane region of the DUF221 conserved region was predicted using TMHMM. The region between two vertical red lines represents the DUF221 conserved region. Identical (100 %), conserved (75–99 %), and blocks (50–74 %) of similar amino acid residues are shaded in dark navy, pink, and cyan, respectively. Identical or similar amino acid residues are shown in lowercase abbreviations at the bottom of the corresponding rows. The transmembrane regions are marked by black lines and called TM1-TM6Back to article page