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Fig. 3 | BMC Plant Biology

Fig. 3

From: Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections

Fig. 3

Effect of biochar on the attractiveness of rice roots to M. graminicola and microscopic observations of giant cells induced in the root system. a Attraction of M. graminicola towards the root tips of rice after root drenching with 1.2 % biochar exudates or water were observed under a Leica stereomicroscope with a DFC400 camera. b Nematodes in the vicinity of the root elongation zone were counted at 9 hpi. The bars represent the mean ± SE of the data from 6 replicates. No significant differences were found (Duncan’s multiple range test at p > 0.05). c Sections of giant cells in the biochar-amended root galls and non-amended root galls were stained with toluidine blue and observed at 7 dpi under an Olympus BX 51 microscope with a ColorView III camera. Multiple sections of 10 galls were evaluated and the figure shows one representative section for each treatment

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