Identification and comprehensive analyses of the CBL and CIPK gene families in wheat (Triticum aestivum L.)

BMC Plant Biology

Table 2 Interactions of TaCIPKs with TaCBLs in yeast two-hybrid assay

	TaCBL1-BD	TaCBL2-BD	TaCBL3-BD	TaCBL4-BD	TaCBL6-BD	TaCBL7-BD	TaCBL9-BD
TaCIPK3-AD	+	+	+	+	-	-	-
TaCIPK5-AD	+	+	-	+	+	+	-
TaCIPK7-AD	-	-	-	-	-	-	-
TaCIPK8-AD	-	-	+	-	-	-	-
TaCIPK10-AD	-	-	-	-	-	-	-
TaCIPK11-AD	-	+	+	-	+	-	+
TaCIPK14-AD	+	+	+	+	+	+	-
TaCIPK15-AD	+ *	+	+	+*	+*	+	-
TaCIPK17-AD	-	+	+	-	-	-	-
TaCIPK19-AD	-	-	-	-	-	-	-
TaCIPK21-AD	-	+	+	-	+	+	-
TaCIPK22-AD	-	-	-	-	-	+	-
TaCIPK24-AD	-	-	-	-	-	-	-
TaCIPK25-AD	+	+	-	-	-	-	-
TaCIPK26-AD	-	+	+	+	-	+	-
TaCIPK27-AD	-	+	+*	-	+	-*	-
TaCIPK28-AD	-	-	-	-	-	-	-
TaCIPK29-AD	-	-	-	-	-	-	-
TaCIPK31-AD	-	+*	+	+	-	+	+*
TaCIPK32-AD	-	+	+	-	-	-	-

The interaction analyses of wheat TaCBL and TaCIPK proteins were performed by Y2H method. TaCIPKs and TaCBLs were respectively cloned to PGAD and PGBK Vector, and then co-transformed into Y187 strains. The transformants containing the target plasmid combinations were grown on selection medium (TDO: SD/–Trp/–Leu/–His/+10 mM 3AT) and indicated as growth (+, interaction) and no growth (−, no interaction). “*” represents the interactions were verified by BiFC assays

ISSN: 1471-2229