Fig. 5

Influence of 5 min UV-B (8 W·m⁻²) irradiation on photosynthesis in Arabidopsis leaves. After irradiation samples were kept either in darkness or under constant light (100 μmol·m⁻²·s⁻¹) for the given time. Day 0 means 1 h after the treatment. Non-irradiated leaf halves served as a control. a Changes in PSII maximal quantum yield (Fv/Fm) during experimental treatment, measured with an imaging fluorometer. The results are the means of measurements for at least 12 different leaves. Statistical significance of the differences between treatments was assessed with one-way ANOVA and the results of this analysis are listed in Additional file 3: Table S3. b Total proteins (upper- Coomassie stained SDS-PAGE) and D1 protein (lower- Western blot with anti-D1 antibodies) in examined leaves. Each well contains proteins extracted from 120 mg of tissue The degradation product of D1 protein is marked with an arrow. c and d Relative expression levels of photosynthesis-related genes (CAB, RBSC1) measured with real-time RT-PCR and normalized for the expression of four housekeeping genes (PDF2, UBC9, UBQ10, SAND). After the specified time period leaves were cut into halves and control and treated halves from 4 different leaves were pooled. Each measurement was repeated at least 3 times. A single dark-adapted overnight control sample from day 0 was used as a reference for calculating relative expression levels. Error bars indicate the standard error