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Fig. 2 | BMC Plant Biology

Fig. 2

From: A small multigene hydroxyproline-O-galactosyltransferase family functions in arabinogalactan-protein glycosylation, growth and development in Arabidopsis

Fig. 2

Substrate specificity of transiently expressed GALT2-6. Detergent permeablized tobacco microsomal membranes obtained from transiently expressed GALT2-6 served as the enzyme source in the GALT reactions. Various peptide and polysaccharide acceptor substrates were tested including: 1) [AO]7, [AO]14, and d[AO]51 which contain 7, 14, and 51 [AO] units, respectively, 2) a chemically synthesized extensin peptide (ExtP) containing repetitive SO4 units, 3) [AP]7 which contains 7 [AP] units, 4) Rhamnogalactan I (RGI) from potato pectin, and 5) RG from soybean pectin. Microsomes obtained from WT tobacco leaves infiltrated with empty pMDC32 vector were used as a negative control and depicted as WT. Enzyme reactions were done in triplicate and mean values ± SE are presented. Asterisks indicate values significantly different from the WT (Dunnett’s test, *P <0.05; **P <0.01)

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