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Table 1 GALT activity and amount of β-Gal-Yariv precipitated AGPs in WT and galt mutants

From: A small multigene hydroxyproline-O-galactosyltransferase family functions in arabinogalactan-protein glycosylation, growth and development in Arabidopsis

Genotype

GALT activity (pmol/h/mg)

β-Gal-Yariv precipitated AGPs (μg/g)

WT

7.10 ± 0.90

13.60 ± 0.75

galt1-1

6.80 ± 0.37

13.30 ± 0.95

galt1-2

7.20 ± 0.65

13.30 ± 0.95

galt2-1

5.53 ± 1.20b

9.91 ± 2.80b

galt2-2

5.90 ± 1.01b

9.28 ± 1.50b

galt3-1

6.08 ± 1.20a

12.10 ± 0.95

galt3-2

5.51 ± 1.01b

12.30 ± 0.80a

galt4-1

6.04 ± 2.20a

12.00 ± 1.10a

galt4-2

5.83 ± 1.50b

11.90 ± 1.20a

galt5-1

5.45 ± 1.10b

7.90 ± 2.10b

galt5-2

4.90 ± 1.50b

8.10 ± 1.20b

galt6-1

5.30 ± 0.44b

10.90 ± 0.59a

galt6-2

5.00 ± 1.71b

10.30 ± 1.54a

galt2galt5

4.64 ± 0.54b

5.63 ± 0.39b

  1. Detergent-solubilized microsomal fractions were used for performing a standard Hyp-GALT assay, and AGPs were extracted, precipitated by β-Gal-Yariv reagent, and quantified from 14-day-old plants. The values are averages of at least two independent experiments from two biological replicates. Letters ‘a’ and ‘b’ denote a significant difference from the wild type (Dunnett’s test, P <0.05; P <0.01) respectively