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Table 1 CWPs identified in sugarcane young culms

From: Cell wall proteome of sugarcane stems: comparison of a destructive and a non-destructive extraction method showed differences in glycoside hydrolases and peroxidases

SUCEST accession numbera

Number of peptidesb

Number of unique peptidesb

Protein score

Femtomole average

S. bicolor homologues

Functional annotation

Extraction method

Proteins acting on polysaccharides

SCCCCL3001B10.b

16; 16

3; 6

4368.653; 1207.559

87.40605

Sb01g010840.1

GH1

ND

 SCJFLR1017E03

7; 8

1; 1

627.7585; 389.633

4.65435

Sb01g010840.1

GH1

ND

 SCEQLB1066E08

11; 5

9; 2

1258.872; 452.9845

6.72165

Sb01g010825.1

GH1

ND

 SCEQHR1082B01

9; 7

8; 6

2256.574; 388.3896

28.182652

Sb02g028400.1

GH1

ND

 SCEZLB1007A09

18; 12

11; 8

4982.336; 2259.83

41.55905

Sb01g008030.2

GH3

ND

SCEQLR1093F09*

12; 18–20; 14

5; 8–6; 5

523.6582; 1309.333 – 3688.09; 10810.11

17.23065 – 50.3928

Sb01g008040.3*

GH3

DT - ND2

 SCCCCL4009F05

20; 14

16; 12

10955.92; 8452.891

156.84746

Sb06g030270.1

GH3

ND

 SCQSAM1030G04

3; 2

3; 2

8506.176; 6709.646

71.61725

Sb06g030270.1

GH3

ND

SCQSRT2031D12

10; 1

7; 9

1169.301; 2716.698

34.5685

Sb03g045490.1

GH17

ND

 SCVPRZ3029G05

3; 2

2; 1

853.3968; 815.915

15.9117

Sb03g040600.1

GH18

ND

SCJLLB2076C12

9; 8

4; 6

3324.314; 1244.686

40.19725

Sb06g021220.1

GH19

ND

SCEZRZ3015E11

8; 5

6; 5

3106.044; 2091.409

55.74035

Sb01g048140.1

GH19

ND

SCCCCL5004G07

8; 8

5; 6

7937.636; 2173.338

76.40835

Sb10g000660.1

GH28

ND

 SCJFRT1007G04

4; 2

1; 1

4752.977; 2164.125

31.240002

Sb10g000660.1

GH28

ND

 SCCCCL6004H07

9; 8

7; 7

633.8843; 542.2719

19.80175

Sb01g040750.1

GH35

ND

 SCVPRZ3029F03

6; 4

4; 4

1035.417; 246.8027

8.5248

Sb03g029700.1

Acyl esterase (homologous to AtPMR5)

ND

 SCSGLR1025E03

5; 2–5; 8

4; 2–4; 8

460.239; 887.9516- 1045.544; 226,6407

19.8042 – 13.1516

Sb02g042780.1

Pectin methylesterase (carbohydrate esterase family 8, CE8)

DT – ND

Oxido-reductases

SCCCRZ1002B03

8; 1

3; 2

708.6577; 447.837

12.9346

Sb01g041770.1

Prx homologous to SbPrx20

DT

SCCCRT1001G12

9; 9–14; 9

5; 6–7; 5

2689.641; 2574.119 – 10308.06; 9888.154

65.36725 – 77.029495

Sb04g008590.1

Prx homologous to SbPrx71

DT - ND

SCCCLB1004B09*

9; 16

4; 4

690.8371; 1079.03

26.908451

Sb10g027490.1*

Prx homologous to SbPrx139

DT

 SCEQRT2030A04*

7; 12

1; 3

306.9711; 658.3382

7.35725

Sb10g027490.1

Prx homologous to SbPrx139

DT

SCCCLR1C03A09

12; 11

8; 7

845.2605; 759.2087

46.113102

Sb09g004650.1*

Prx homologous to SbPrx115

DT

 SCCCLR1C05G08*

11; 11

5; 8

1494.011; 1461.387

66.7759

Sb03g024460.1*

Prx homologous to SbPrx65

DT

 SCRLAD1042E05

6; 4–5; 2

1; 2–1; 1

2528.694; 933.4598 – 873.3041; 1444.467

17.7972 – 10.54785

Sb09g002740.1*

Prx homologous to SbPrx108

DT – ND

 SCVPRZ2035F03*

11; 8–9; 5

6; 6–4; 3

2417.947; 1151.436 – 1401.302; 1033.822

42.28915 – 17.264

Sb09g002740.1

Prx homologous to SbPrx108

DT - ND

 SCVPLB1020D03

2; 8

2; 7

372.5895; 854.9581

23.364399

Sb03g046760.1

Prx homologous to SbPrx68

DT

SCEPRZ1011A06*

7; 11–12; 6

3; 5–4; 3

866.9835; 940.5853 – 5970.014; 1026.785

17.7399 – 45.46655

Sb03g010250.1*

Prx homologous to SbPrx54

DT - ND

SCCCAD1001B08

3; 3

1; 1

9547.608; 3981.619

Identified

Sb03g010740.1

Prx homologous to SbPrx55

ND

SCJFRZ2013F04

7; 1

1; 1

23916.97; 9892.667

7.78455

ND

SCJLRT1019B02

9; 6

1; 1

16559.67; 6310.499

14.36725

ND

SCEQRT1024D03

1; 1

3; 2

16709.58; 4972.862

60.093697

Sb03g010740.1

Prx homologous to SbPrx55

ND

SCCCAD1001C08

6; 5

3; 4

7855.956; 13571.95

28.358952

Sb02g042860.1

Prx homologous to SbPrx47

ND

 SCQSST3114C09

5; 8

5; 4

2082.782; 583.5138

16.338501

Sb01g031740.1

Prx homologous to SbPrx14

ND

SCBFFL4112F05

2; 3

1; 2

1435.365; 4620.795

35.5025

Sb06g018350.1

Blue copper binding protein (plastocyanin)

DT

SCRFHR1006G03

3; 2

2; 1

391.4637; 1712.47

1.22305

Sb01g010510.1

Blue copper binding protein (plastocyanin)

DT

 SCJLLR1104H07

3; 3

3; 3

912.4164; 417.4785

15.684

Sb07g011870.1

blue copper binding protein (plastocyanin)

ND

 SCEPAM1021H07

8; 3

3; 3

924.3445; 347.9209

12.423151

Sb10g027270.1

Multicopper oxidase

ND

Proteins related to lipid metabolism

SCCCAM2002F12

4; 5–4; 5

1; 1–1; 1

716.9828; 1069.318 – 5961.475; 9251.332

19.3443 – 115.4035

Sb03g038280.1

LTP

DT – ND

SCBFLR1046E09

5; 6–4; 5

1; 1–1; 1

816.9942; 1488.674 – 14868.01; 16290.19

34.07175 – 242.60735

Sb03g038280.1

LTP

DT - ND

SCVPRZ2039B03

5; 6–4; 5

1; 1–1; 1

816.9942; 2065.907 – 14868.01; 16290.19

Identified - identified

DT - ND

SCVPRZ2041C11

5; 6–4; 5

1; 1–1; 1

902.0069; 1488.674 – 18617.13; 24758.37

8.77335 - identified

DT - ND

SCCCLR1072C06

3; 2–6; 5

1; 1–1; 1

243.1648; 2495.928 – 35001.54; 23317.92

6.5461 – 132.9761

Sb08g002700.1

LTP

DT - ND

SCRFLR1012A10

3; 2–7; 5

1; 1–1; 1

345.6155; 2314.583 – 35158.61; 23317.92

Identified - identified

DT – ND

SCEPRT2047G01

10; 5

1; 1

35204.81; 23919.76

250.87096

ND

SCEZLR1031G07

5; 4

1; 1

34256.59; 23296.33

Identified

ND

SCRUSB1064D08

9; 5

1; 1

35280.34; 23317.92

Identified

ND

SCEPLB1044H04

3; 4–3; 4

1; 1–1; 1

3924.14; 3159.724 – 1548.66; 926.7365

189.36455 – 46.1548

Sb01g049830.1

LTP

DT – ND

SCEZLB1006F09

3; 4–3; 2

1; 1–1; 1

6772.019; 11919.76 – 8845.361; 13343.73

194.30121 – 146.9349

Sb08g002670.1

Protease inhibitor/seed storage/LTP family

DT - ND

 SCCCLR1048F06 - SCCCLR1048F06

10; 13–5; 4

1; 1–1; 2

91432.66; 77846.23 – 176534.4; 124470.6

318.6974 – 352.27365

Sb08g002690.1

Protease inhibitor/seed storage/LTP family

DT - ND

SCBGLR1114E07

5; 5

2; 2

145690.4; 125905.7

588.4425

ND

SCCCCL3004H07.b

3; 3

2; 2

145392.5; 124459

Identified

ND

SCVPHR1092G06

4; 4

2; 2

145392.5; 124470.6

Identified

ND

SCUTST3131G03

3; 6–4; 3

2; 1–1; 1

6793.345; 3745.457 – 21630.89; 20854.45

150.85635 – 109.76019

Sb08g002690.1

Protease inhibitor/seed storage/LTP family

DT – ND

 SCCCCL3001E03.b*

5; 7

2; 3

3263.44; 1945.298

39.72605

Sb01g033830.1*

LTP

ND

SCJFRZ2033G07

4; 3

1; 1

26826.32; 13939.5

8.15625

Sb08g002700.1

LTP

ND

SCRUFL4024B04

4; 3

1; 1

26805.06; 13987.92

202.91615

ND

 SCCCRZ1001H02

3; 3

1; 1

7741.618; 3045.282

70.59645

Sb03g039880.1

LTP

ND

SCCCRZ2002G09

5; 5

2; 2

20532.6; 6676.209

49.378

Sb06g016170.1

LTP

ND

SCQSFL3039E08.b

5; 5

2; 2

22572.43; 7334.285

20.2533

ND

SCCCLR1024C05*

6; 3

1; 1

11552.57; 5130.042

5.59605

Sb08g002660.1*

Protease inhibitor/seed storage/LTP family

ND

SCCCLR1076D05

6; 5

1; 1

16880.65; 8523.134

129.08115

ND

SCEPLB1044H11*

7; 3

1; 1

11788.22; 5527.095

13.30225

ND

SCCCLR2C03F01

3; 3

1; 1

9426.873; 6414.531

78.86415

Sb08g002670.1

Protease inhibitor/seed storage/LTP family

ND

 SCCCRT1003B03

6; 4

2; 3

722.451; 408.6039

26.09375

Sb10g003930.1

GDSL lipase

ND

Proteases

 SCBGLR1023G11

6; 8

5; 8

553.142; 705.4267

24.15155

Sb04g029670.1

Asp protease, peptidase A1

DT

 SCBGLR1097G03

4; 6

3; 3

1475.072; 7045.55

168.19795

Sb05g027510.1

Asp protease, peptidase A1

DT

 SCMCLR1123H12

6; 7–3; 2

3; 3–2; 1

1722.723; 4799.767 – 598.7939; 1717.224

122.60135 – 52.145752

Sb05g027510.1

Asp protease, peptidase A1

DT - ND

 SCQGST1032H01

11; 14

8; 7

653.9818; 997.1608

45.5457

Sb05g027510.1

Asp protease, peptidase A1

DT

 SCQGSB1083B11

8; 5

5; 4

4851.335; 4946.649

47.901802

Sb02g041760.1

Asp protease, peptidase A1

ND

 SCRLRZ3042B09

9; 6

5; 3

390.5894; 367.0505

7.24305

Sb03g026970.1

Asp protease, peptidase A1

ND

 SCVPLR2012E01

3; 3–4; 3

2; 2–2; 2

1075.957; 4197.95 – 23533.5; 11935.54

147.1347 – 160.93646

Sb01g044790.1

Asp protease/Taxi _N/Taxi_C

DT - ND

 SCVPRZ2038B09

3; 4–4; 2

2; 3–4; 2

1194.285; 2283.918 – 6719.425; 1320.476

61.6057 – 103.41875

Sb01g044790.1

Asp protease/Taxi _N/Taxi_C

DT - ND

SCCCST1004B07

11; 8

11; 8

4096.934; 3149.51

44.2912

Sb01g013970.1

Ser protease (subtilisin family, peptidase S8/S53)

ND

 SCJFRZ2011B07

5; 4

4; 3

2390.547; 1211.642

25.90875

Sb06g016860.1

Ser protease (subtilisin family, peptidase S8/S53)

ND

SCCCLR1022B11*

7; 5

6; 6

1017.185; 492.4018

20.1544

Sb06g030800.1*

Cys protease, (papain family, peptidase C1A)

ND

Proteins with interaction domains (with proteins or polysaccharides)

SCJFLR1013A04

4; 4

1; 1

3741.598; 4709.589

31.54085

Sb05g026650.1

Ser protease inhibitor (Bowman-Birk)

DT

SCRUFL3062D08 - SCRUFL3062D08

5; 5–4; 4

1; 1–1; 1

2784.365; 4868.514 – 11731.47; 8790.486

45.9138 – 44.6385

DT - ND

Signaling

 SCRUAD1063C06

4; 2

4; 4

5824.59; 1313.647

55.09195

Sb09g000430.1

Leucine-rich repeat (LRR) receptor kinase

ND

Miscellaneous proteins

SCEZRZ1014C04*

6; 5–4; 8

2; 2–1; 1

6025.488; 9344.188 – 18335.89; 4427.775

79.66205 – 67.5792

Sb03g039330.1*

Thaumatin

DT - ND

SCCCLR2003G06

4; 4

1; 2

1364.424; 533.9335

18.352499

Sb08g018720.1

Thaumatin

ND

SCUTLR1037F02

3; 4

1; 2

1083.055; 546.6533

Identified

ND

SCCCSD1003E02

3; 2

1; 1

2326.563; 4054.495

18.28735

Sb08g022410.1

Thaumatin

ND

SCRUHR1076B06

3; 2

1; 1

3641.288; 5434.818

2.588

Sb08g022410.1

Thaumatin

ND

SCVPRT2073B04

4; 4

2; 2

2289.087; 18490.86

87.17195

Sb08g022420.1

Thaumatin

ND

SCBGRT1047G10

6; 7

4; 6

3131.626; 2684.819

52.720253

Sb02g004500.1

Germin (cupin domain)

ND

SCCCLR2C02D04

3; 4

3; 3

9021.729; 14936.96

149.43965

Sb09g004970.1

Germin (cupin domain)

ND

 SCCCRZ1C01H06

13; 1–12; 14

4; 3–7; 6

3376.122; 3105.723 – 10082.07; 3506.265

55.5037 – 33.9362

Sb08g001950.1

Nucleoside phosphatase

DT - ND

 SCJLRT3078H06

6; 2

3; 1

1286.289; 1236.944

45.866447

Sb05g025670.1

Dirigent protein

DT

 SCVPRT2073B08

6; 4

4; 1

333.12; 1046.494

19.9186

Sb10g001940.1

SCP-like extracellular protein

ND

Unknown function

 SCCCCL4009G04*

11; 1–8; 8

4; 4–3; 3

1383.823; 3928.575 – 15270.52; 16670.59

126.7531 – 141.14679

Sb01g004270.1*

Unknown function (DUF642)

DT - ND

 SCSGLR1084A12*

12; 14–10; 9

6; 6–6; 5

6772.538; 5277.542 – 18024.07; 13067.63

158.43881 – 150.2402

Sb01g004270.1

Unknown function (DUF642)

DT - ND

 SCCCLB1001G04

7; 3

5; 3

314.0911; 273.5209

9.64495

Sb03g027650.1

Unknown function (DUF642)

DT

 SCVPLR2027A11

5; 4–5; 3

5; 4–2; 1

2870.491; 1609.635

34.981

Sb07g026630.1

Unknown function (DUF568)

ND

 SCCCRZ3002G10*

4; 7–6; 5

1; 1–1; 2

844.0264; 313.8415 – 4025.449; 1307.088

3.07575 – 35.7293

Sb01g031470.1*

Homologous to phloem filament protein 1 (Cucurbita phloem)

DT - ND

 SCEZRT2018F03*

4; 6

1; 1

675.4673; 446.4631

5.89995

Sb01g031470.1

Homologous to phloem filament protein 1 (Cucurbita phloem)

DT

 SCEZLB1013B06

14; 15

8; 11

5254.302; 4812.086

137.18835

Sb10g001440.1

Homologous to phloem filament protein 1 (Cucurbita phloem)

DT

SCSFST1066G10

5; 5–6; 5

1; 1–1; 1

718.6985; 2383.583 – 15310.67; 5735.458

102.53975 – 192.7034

Sb08g018710.1

Expressed protein

DT - ND

 SCRUFL4024B08.b

3; 6

1; 2

5551.887; 12727.94 – 52010.09; 28346.53

120.5459 – 288.07706

Sb08g018710.1

Expressed protein

DT - ND

 SCCCRZ2004B02*

8; 8

1; 1

9551.813; 4751.091

43.9473

Sb03g000700.1*

Expressed protein

ND

SCCCLR1079C11

7; 4

6; 4

3527.088; 4063.023

33.617348

Sb04g011100.1

Expressed protein

ND

SCAGLR2011E04/SCEPAM2057B02

3; 3

1; 1

11178.66/11961; 8954.521/7215.72

identified

Sb08g003040.1

Expressed protein (stress responsive alpha/beta barrel)

ND

SCEPLR1051E09

3; 3

1; 1

11178.66; 7215.72

28.171349

ND

  1. aBold letters indicate that the ESTs share common sequences. Full length ESTs are in italics. Stars (*) indicate the proteins also identified in the cell wall proteome of sugarcane cell suspension cultures [15]
  2. bSemicolons separate data from different biological repeats. Dashes separate data from different extraction methods (DT, then ND)