Fig. 2

Variability in PIN1 expression domain in different lineages in the root tip. a and b The primary root tips of two individual 4 dag seedlings labelled with anti-PIN1 (green channel) and DAPI (blue channel). CSC—columella stem cell, c2—the second columella tier, c3- the third columella tier, epid—epidermis, c—cortex, en—endodermis, prc—pericycle, vsc—vasculature, px—protoxylem. Coloured triangles—the end of the expression domain in the respective layer. Bars = 50 μm. c A general scheme of stable and variable parts of the PIN1 expression pattern in the root tip of A. thaliana. PIN1 expression domain is on the left: dark grey—the stable part of the PIN1 protein domain for the root meristem of a 4 dag seedling, grown on MS medium (see Methods); light grey is its variable part. On the right, stable and variable features of PIN1 polarization are shown. RW—rootward polarity, RWL—rootward and lateral polarity, RWLI—rootward and inner lateral polarity, NP—nonpolar