Fig. 4

Male gametophytic ploidy distribution in tetraploid Arabidopsis using pWOX2-CENH3-GFP. a-d Late uninuclear microspores from tetraploid Arabidopsis expressing pWOX2-CENH3-GFP. Images are processed z-stacks. The microspores either contain the diploid number a or an aneuploid number of centromeric GFP signals b-d. e Frequency distribution of CENH3-GFP signals in nuclei of late uninuclear microspores isolated from diploid and tetraploid Arabidopsis controls. Error bars represent standard deviation (of three independent analyses of at least 100 spores). f-h Representative images of male meiotic chromosome spreads at the start of MII in a wild type tetraploid line showing either a balanced f or an unbalanced g and h segregation of homologous chromosomes in MI. In some cases, one or more lagging chromosomes are observed h. Scale bars, 10 μm