Fig. 4

Expression of TaHKT2;2 genes under non-treated and saline conditions. a Na⁺ concentration of tissue samples for qRT-PCR analysis. Na⁺ ion concentrations were measured in triplicate from four biological replicates for untreated (control) and 200 mM NaCl treated tissue samples. b Agarose gel electrophoresis showing sub-genome specificity of primer pairs used in qRT-PCR of group II HKT gene members using NT lines. Each line is in duplicate (c). Bar graph representing fold change estimated using the ∆∆C_T method. The error bars represent possible range of relative quantity values, RQ_max and RQ_min, defined by the standard error of the ∆C_T s qRT-PCR for transcript analysis of group II HKT gene family members in wheat (Chinese Spring) using cDNA from root (R), sheath (S) and leaf (L) tissue samples untreated and treated for 72 h in 200 mM NaCl. The internal control genes TaActin was used to normalize for variability in initial RNA template for each reaction. The grey shaded region within the graph highlights the fold range that has no significant difference