Skip to main content

Table 2 Gene specific primers and PCR annealing temperatures to amplify genomic and cDNA sequence from members of the TaHKT2;2 gene family and qRT-PCR for TaHKT2;1 and TaHKT2;2 genes

From: A comparative gene analysis with rice identified orthologous group II HKT genes and their association with Na+ concentration in bread wheat

 

Forward primer

Reverse Primer

Gene

Primer

Primer sequence (5’-3’)

Primer

Primer sequence (5’-3’)

Annealing Temperature °C

TaHKT2;2 7AL-1

2;2AF1

CCATCTATCTAACTCCAATGACTG

2;2AR1

CTCTCAGTGCCCATACCAT

54

TaHKT2;2 7AL-1

2;2AF2

TCAATACCATGCTCTTCACG

2;2AR2

AGACGCTCTGCTTCTCTGC

55–50

TaHKT2;2 7AL-1

2;2AF3

GGGGCAAACAAGAGAAGAA

2;2ADR1

CTAGCTCCTCTGCCTGTG

55–50

TaHKT2;2 7BL-1

2;2CF1

CTATCTAACTCCAATGCCTATC

2;2BR1

GCAAGTGGTTGAAACTCACAC

60–50

TaHKT2;2 7BL-1

2;2BF2

CTGTTGGCATACATGGTATC

2;2BR2

ATGGACAGTCCTACGTTCATA

65–55

TaHKT2;2 7BL-1

2;2BF3

CCGTTGCCATCACACTCTTG

2;2ADR1

CTAGCTCCTCTGCCTGTG

60–50

TaHKT2;2 7DL-1

2;2ADCF1

CACACTGCCATCTATCTAACTC

2;2DR1

ACAGTCTTCTCTTGTTCGCT

55–50

TaHKT2;2 7DL-1

2;2DF2

TGCTTTTCTCCATACCCA

2;2ADR1

CTAGCTCCTCTGCCTGTG

55–50

TaHKT2;1 7AL-1

2;1AF1

TCGGCTCTTATCAGAACACA

2;1AR1

CCACACGTTGATAGATAATGTC

55

TaHKT2;1 7AL-1

qPCR2;1AF1

ATGTCCCCTGCCATTGTAGAAT

qPCR2;1AR1

CGTGTTCTCATTGGTGGTTTTACTG

60

TaHKT2;1 7AL-1

qPCR2;1BF1

TGCGTTTTGCTAATTTGCCTG

qPCR2;1BR1

GATAAGAGCTGAGCCCATCCAAG

60

TaHKT2;1 7DL-1

qPCR2;1DF1

ACTGTTTTTCTCTCCTCAACGCTT

qPCR2;1DR1

TGCCTTTTGTGCTCGCTTC

60

TaHKT2;2 7AL-1

qPCR2;2AF1

GATATGGGCACTGAGAGGACTATGA

qPCR2;2AR1

AAACAGCATTTTATTCAGCGAGAT

60

TaHKT2;2 7BL-1

qPCR2;2BF1

GCTGCTTGAACTGGAATGCG

qPCR2;2BR1

GTGTTCTGTGATGCCCCTCTTGT

60

TaHKT2;2 7DL-1

qPCR2;2DF1

GATTCACTTGTCCTATTTTGTTGTCG

qPCR2;2DR1

GCAGGGAAACAAACATCTCTCTG

58

TaActin

TaActin_qR

TGGCACCCGAGGAGCACCCTG

TaActin_qR

GCGACGTACATGGCAGGAACA

60

GAPDH

GAPDHF

CGCCAGGGTTTTCCCAGTCACGAC

GAPDHR

TCAC ACAGGAAACAGCTATGAC

60

TaEFA

TaEFA_qF

GATTGGCAACGGCTACG

TaEFA_qR

CGGACAGCAAAACGACC

60