| Forward primer | Reverse Primer | |||
---|---|---|---|---|---|
Gene | Primer | Primer sequence (5’-3’) | Primer | Primer sequence (5’-3’) | Annealing Temperature °C |
TaHKT2;2 7AL-1 | 2;2AF1 | CCATCTATCTAACTCCAATGACTG | 2;2AR1 | CTCTCAGTGCCCATACCAT | 54 |
TaHKT2;2 7AL-1 | 2;2AF2 | TCAATACCATGCTCTTCACG | 2;2AR2 | AGACGCTCTGCTTCTCTGC | 55–50 |
TaHKT2;2 7AL-1 | 2;2AF3 | GGGGCAAACAAGAGAAGAA | 2;2ADR1 | CTAGCTCCTCTGCCTGTG | 55–50 |
TaHKT2;2 7BL-1 | 2;2CF1 | CTATCTAACTCCAATGCCTATC | 2;2BR1 | GCAAGTGGTTGAAACTCACAC | 60–50 |
TaHKT2;2 7BL-1 | 2;2BF2 | CTGTTGGCATACATGGTATC | 2;2BR2 | ATGGACAGTCCTACGTTCATA | 65–55 |
TaHKT2;2 7BL-1 | 2;2BF3 | CCGTTGCCATCACACTCTTG | 2;2ADR1 | CTAGCTCCTCTGCCTGTG | 60–50 |
TaHKT2;2 7DL-1 | 2;2ADCF1 | CACACTGCCATCTATCTAACTC | 2;2DR1 | ACAGTCTTCTCTTGTTCGCT | 55–50 |
TaHKT2;2 7DL-1 | 2;2DF2 | TGCTTTTCTCCATACCCA | 2;2ADR1 | CTAGCTCCTCTGCCTGTG | 55–50 |
TaHKT2;1 7AL-1 | 2;1AF1 | TCGGCTCTTATCAGAACACA | 2;1AR1 | CCACACGTTGATAGATAATGTC | 55 |
TaHKT2;1 7AL-1 | qPCR2;1AF1 | ATGTCCCCTGCCATTGTAGAAT | qPCR2;1AR1 | CGTGTTCTCATTGGTGGTTTTACTG | 60 |
TaHKT2;1 7AL-1 | qPCR2;1BF1 | TGCGTTTTGCTAATTTGCCTG | qPCR2;1BR1 | GATAAGAGCTGAGCCCATCCAAG | 60 |
TaHKT2;1 7DL-1 | qPCR2;1DF1 | ACTGTTTTTCTCTCCTCAACGCTT | qPCR2;1DR1 | TGCCTTTTGTGCTCGCTTC | 60 |
TaHKT2;2 7AL-1 | qPCR2;2AF1 | GATATGGGCACTGAGAGGACTATGA | qPCR2;2AR1 | AAACAGCATTTTATTCAGCGAGAT | 60 |
TaHKT2;2 7BL-1 | qPCR2;2BF1 | GCTGCTTGAACTGGAATGCG | qPCR2;2BR1 | GTGTTCTGTGATGCCCCTCTTGT | 60 |
TaHKT2;2 7DL-1 | qPCR2;2DF1 | GATTCACTTGTCCTATTTTGTTGTCG | qPCR2;2DR1 | GCAGGGAAACAAACATCTCTCTG | 58 |
TaActin | TaActin_qR | TGGCACCCGAGGAGCACCCTG | TaActin_qR | GCGACGTACATGGCAGGAACA | 60 |
GAPDH | GAPDHF | CGCCAGGGTTTTCCCAGTCACGAC | GAPDHR | TCAC ACAGGAAACAGCTATGAC | 60 |
TaEFA | TaEFA_qF | GATTGGCAACGGCTACG | TaEFA_qR | CGGACAGCAAAACGACC | 60 |