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Fig. 3 | BMC Plant Biology

Fig. 3

From: Functional and evolutionary analyses of the miR156 and miR529 families in land plants

Fig. 3

Phenotypic, expression, and RACE analyses of Arabidopsis 35S::AqcMIR529 plants. a Morphology of 25-day-old plants (Col-0 or wild type, 35S::MIR156a, spl9;spl15, and 35S::AqcMIR529). Scale bar represents 1 cm. b Stem–loop pulsed RT-PCR to detect AqcMIR529 precursor and some AtSPL transcripts in Arabidopsis leaf tissues. Reactions without RT (−RT) and without cDNA (−) were used as negative controls. Numbers between brackets indicate PCR cycles. Actin-2 was used as an internal control. c Modified 5′-RACE analyzes of cleaved SPL15 transcripts in 35S::AqcMIR529 leaf tissues. The 5′-ends of the SPL15 cleavage products preferentially map to miR156

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