Lignin biosynthesis in wheat (Triticum aestivum L.): its response to waterlogging and association with hormonal levels

Table 2 Relative transcript levels of lignin biosynthesis genes highly expressed in different tissues of wheat^w

Gene	FB	FS	PE	IN-1	IN-2&3	IN-4
PAL6	0.83 ± 0.07^xd^y	9.05 ± 0.80c	89.25 ± 19.53a	54.50 ± 6.71b	54.57 ± 11.98b	33.66 ± 6.98b
C4H1	0.38 ± 0.03d	0.91 ± 0.04c	2.68 ± 0.40b	3.67 ± 0.61b	9.57 ± 1.20a	11.22 ± 3.71a
4CL1	1.00 ± 0.09d	2.04 ± 0.08c	7.61 ± 1.10b	7.13 ± 0.57b	12.51 ± 2.02a	16.84 ± 2.83a
HCT1	0.58 ± 0.03d	0.70 ± 0.06d	2.53 ± 0.54b	1.54 ± 0.20c	3.09 ± 0.40b	5.42 ± 0.60a
C3H1	0.12 ± 0.03e	0.21 ± 0.03de	1.24 ± 0.06a	0.87 ± 0.16b	0.55 ± 0.12c	0.46 ± 0.03 cd
CCoAOMT1	1.52 ± 0.17e	3.32 ± 0.58d	32.73 ± 5.28a	11.98 ± 2.51c	19.56 ± 3.51bc	21.89 ± 4.77b
CCR1 ^z	11.11 ± 0.21a	6.64 ± 0.36b	0.29 ± 0.03f	1.59 ± 0.31c	0.78 ± 0.02d	0.45 ± 0.07e
CCR2	0.17 ± 0.02e	1.56 ± 0.07d	3.77 ± 0.78c	5.93 ± 0.29b	8.44 ± 0.85a	7.82 ± 1.09a
CCR5 ^z	13.49 ± 1.10a	9.33 ± 0.21b	0.93 ± 0.09d	2.76 ± 0.66 cd	2.85 ± 0.36 cd	3.54 ± 0.63c
F5H2	0.84 ± 0.04e	1.39 ± 0.04d	9.20 ± 1.67a	6.09 ± 0.97ab	3.38 ± 1.05c	4.23 ± 0.84bc
COMT2	15.51 ± 1.13b	36.04 ± 4.20a	16.09 ± 2.86b	31.56 ± 4.40a	14.18 ± 1.64b	12.63 ± 0.67b
CAD2	1.32 ± 0.07b	2.05 ± 0.08a	0.16 ± 0.00c	0.95 ± 0.22b	2.02 ± 0.17a	2.55 ± 0.29a

^wTranscript levels in different tissues of cv. Harvest were expressed relative to that of 4CL1 in flag leaf, which was arbitrarily set to value of 1
^xData are means of 2 to 3 independent biological replicates ± SE
^yMeans followed by different letters within each gene show statistically significant difference at P < 0.05
^zGenes highly expressed specifically in the leaf tissues
FB flag leaf blade, FS flag leaf sheath, PE peduncle, IN-1 the first internode, IN-2&3 the second and third internode, IN-4 the fourth internode (see the Methods section for numerical designation of internodes)

ISSN: 1471-2229