Fig. 3

Some flg22-induced responses downstream of calcium transients are partially attenuated a Fourteen-day-old seedlings were collected at the indicated time points after flg22 (100 nM) treatment and proteins extracted for western blot analysis with an antibody that recognizes phosphorylated (i.e. activated forms of) MAPKs. The identities of the three MAPK bands are marked on the right. The experiment was repeated thrice with similar outcome. b Reactive oxygen species (ROS) accumulation was assessed in 24 leaf disks per genotype over 120 min. The basal luminescence (relative light units, RLU) of each disk was monitored for 6 min (before treatment) and used to normalize the fold change in RLU after flg22 treatment (marked with arrow). Error bars depict the 95 % confidence intervals. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparison post-test (The different alphabets denote groups that are considered statistically different, p < 0.05). c Growth inhibition of cce2/cce3 mutants compared to the pMAQ2 parental line. Seedlings were placed on agar media without (−) or containing flg22 (1 μM) and the root length measured after 14 days. Left panels show the absolute root lengths of a representative experiment (n = 22–23 seedlings). Right panels are the percent inhibition consolidated from 3 independently performed experiments (n = 67–69 seedlings). Statistical significance was analyzed using two-way ANOVA with Bonferroni post-test (** = p < 0.0001, ns = not significant)