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Table 1 Number of reads sequenced and mapped with Tophat. The number of unique mapping reads plus multimapping reads equals the total number of alignments. CK, H2O and IAA stand for imbibed tobacco seeds and control respectively; Numbers 0, 72 and 156 stand for germinated time

From: Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L.

Sample

Seed category

Total reads

Total mapped reads

Unique mapping reads

Reads mapped in proper pairs

Untreated-0

Dormancy

39,344,236

28,566,421(72.61 %)

24,229,292(61.58 %)

25,845,450

 H2O-0

Imbibition

39,795,012

35,183,360(88.41 %)

21,537,407(66.58 %)

29,258,962

 IAA-0

Dormancy

48,297,176

38,392,739(79.49 %)

32,439,112(67.17 %)

34,779,672

Untreated-72

Germination

33,792,314

30,897,092(91.43 %)

26,584,380(78.67 %)

24,978,136

 H2O-72

Germination

35,593,768

31,962,893(89.80 %)

27,456,500(77.14 %)

26,621,694

 IAA-72

Dormancy

32,459,006

28,789,591(88.70 %)

25,040,225(77.14 %)

23,209,710

 IAA-156

Germination

33,769,804

29,877,097(88.47 %)

25,821,822(76.46 %)

24,613,498