Fig. 4
From: Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases
In vitro Labeling Assay of Wild-type and Mutated MPK Substrates. a Thiophosphorylation assays of native (Wt) and phosphosite mutant (SA) forms of FLAG-tagged MPK substrates AT1G78150, AT2G26530, AT3G11330 and AT4G38710 in the presence of Bn-ATPγS as cofactor and using either AS-MPK3 or Wt-MPK3 as a negative control. b Same as in (A) but using AS-MPK6 and Wt-MPK6. c Same as in (a) but using AS-MPK4 and Wt-MPK4