Skip to main content
Fig. 2 | BMC Plant Biology

Fig. 2

From: Revisiting chromatin binding of the Arabidopsis UV-B photoreceptor UVR8

Fig. 2

UVR8 does not bind to the MYB12 promoter. a UVR8 chromatin association in wild-type plants (WT = Ws/Pro HY5 :Luc+), a uvr8 mutant (uvr8-7/Pro HY5 :Luc+) and a UVR8-overexpression line (UVR8 OX = Ws/Pro HY5 :Luc + Pro 35S :UVR8). ChIP-qPCR was performed using an anti-UVR8(410–424) antibody for immunoprecipitation. b Chromatin association in HY5-YFP expressing plants (hy5-1/Pro 35S :HY5-YFP), a YFP-UVR8 line (uvr8-7/Pro 35S :YFP-UVR8) and hy5-1 and uvr8-7 mutant controls. ChIP-qPCR was performed using an anti-GFP antibody for immunoprecipitation. c HY5 chromatin association in wild-type plants (WT = Ws/Pro HY5 :Luc+), a UVR8 overexpression line (UVR8 OX = Ws/Pro HY5 :Luc + Pro 35S :UVR8) and hy5 hyh mutant control (hy5-ks50 hyh-1/Pro HY5 :Luc+). ChIP-qPCR was performed using an anti-HY5 antibody. a, b, c Chromatin association was analysed by ChIP-qPCR for the MYB12 promoter (Pro MYB12 ), ACTIN2 and an intergenic control region between the At4g26900 and At4g26910 genes. The analysed chromatin was the same as in the experiments presented in Fig. 1. Error bars represent SDs of three technical replicates

Back to article page