Table 4 Primers used for qRT-PCR analysis and candidate loci sequencing
Primer name | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) |
|---|---|---|
qRT-PCR primers | Â | Â |
qRT-5730A | CTGGATCGCCCATTTCACCT | CCCATGTAACTCTGTGCCTGA |
qRT-5760A | TCTCATGGGTTACGGTTGTGG | TTCAAGTTTGTGTCTGATTTCTGT |
qRT-5780A | CTCACCTCATTTGTGCCCCA | ATCACTTGCCCTGTCGTCTC |
qRT-5870A | ATTGGCGGTGAAATCCCACA | AACCATAACCCACGAGCACC |
qRT-6550A | TTTAGTGCAGAAATGGGTTGTGT | CACTTGTCCTGTCGTCTCGT |
qRT-8390A | TCAGATGCTATTAGATTCAGACCTC | GTGGGAGACCTAACAGTGAGC |
qRT-8410A | AGTTCTCAGTATCCAGAGTAGCCT | AGAAATTGGTCCTTCGAGATGGT |
EFα1 | CCACCAATCTTGTACACATCC | AGACCACCAAGTACTACTGCAC |
Sequencing primers | ||
Cf-4/9-1 | TTCGTAGACCAAATTAGCT | CTAGTTTGACCAATGTGAAG |
Cf-4/9-2 | TGGTCATTGATCTTCTAA | ACAGTTGGATTAGTAGGATTAC |
Cf-4/9-3 | AAGGTGTACCTTATAGCAT | GCGTTCGAGTGTTTTCGGGGAA |
S5870A | AACAAAAGTCTTAATTATTTATA | ACCAATTTCCCAGTTCTCGATA |
S5870B | GCTTATTCCAGTCCAATT | CACTTAATGCATTGGCAAAAAGA |