Population genetic analysis of a medicinally significant Australian rainforest tree, Fontainea picrosperma C.T. White (Euphorbiaceae): biogeographic patterns and implications for species domestication and plantation establishment

Table 1 Characterization of eleven microsatellite loci isolated from 218 individuals of Fontainea picrosperma

Locus GenBank	Repeat motif	Primer sequences (5′–3′)	Size range (bp)	PIC	N _A	H _O	H _E	F _IS
FP21 KC759358	(TA)₁₃	F: TCACTGAATTCGCTTGGTTG R: TGCAAATACCAGAAGTGCCA	194–204	0.596	6	0.532	0.664	0.000
FP32 KC759359	(GT)₈	F: CTGGCTTGCATTTGCTTGTA R: TGCTAAACTTCAAGGGCTTAGG	190–192	0.329	2	0.339	0.416	−0.034
FP39 KC759362	(GA)₁₅	F: CTGCACGACAAGAAAACTCG R: TGAGTCAATATTGTAAGGGAATTATGA	203–213	0.293	3	0.280	0.325	−0.004
FP40 KC759363	(TG)₁₆	F: TTCTCGTCCTCTACTGGGCT R: CCCTACCTTTCCCACTCACA	134–152	0.455	6	0.550	0.551	−0.096
FP44 KC759364	(AT)₇	F: TGAAGCTAATTGCTTGATCTTCC R: GGGTATTTATTTTCTTGTTTGTTTCC	112–122	0.390	5	0.459	0.505	−0.117
FP47 KC759365	(TC)₇	F: CCTAAAAGTGCCCTTTGGCTA R: TGTGACTTTCCATGCTCCAG	238–242	0.284	3	0.307	0.338	−0.192
FP49 KM213753	(GA)₈	F: TTTATACAACCACCAGTCGCC R: CACCTTCACTGAAATTCTCTTCTTC	171–175	0.479	3	0.468	0.537	0.013
FP56 KM213754	(TA)₁₄	F: CAGGGCTTAGAATCGGGTGT R: TCACATCCTAGGTCCGTTCAC	258–270	0.776	7	0.391	0.806	0.390
FP59 KM213755	(AT)₁₁	F: TCCCTCCTGTTAAGACTGTTACA R: CCTTCACCATCAATCAGCCG	210–218	0.163	2	0.143	0.179	0.128
FP62 KM213756	(TC)₁₁	F: TGAAAATGCTGACCAAATATGTGA R: AGTTTCCCAGGATCCCACAT	271–273	0.375	2	0.468	0.501	−0.086
FP64 KM213757	(GAC)₁₁	F: ACGGTGAAGACGATGATGGT R: CGTGTGTTACCTCTTCTTCAGC	108–129	0.581	6	0.385	0.631	0.075
			Mean	0.429	4.1	0.393	0.496	0.007

Samples were collected from the Atherton Tablelands, Australia from seven locations shown in Fig. 1. PIC polymorphic information content; N _A number of alleles; H _O observed heterozygosity; H _E expected heterozygosity; F _IS inbreeding coefficient

ISSN: 1471-2229