Table 1 List of primers used in this study
Primer | Sequence (5′ to 3′) | Product size (bp) | Description |
|---|---|---|---|
GMD1-3O | TGGTCCACAACTACGCCAACGGGCTCAAG | 730 | 3′-RACE for Sjgmd1 |
GMD1-3I | CGACGCTCAGAAGGTGCT | ||
GMD2-3O | TTCCACCCGGACGACTGCCCGTCGGAC | 491 | 3′-RACE for Sjgmd2 |
GMD2-3I | CGACACCCGGGAGCGCATGTCCAAC | ||
GMD1-5O | GGGACTGCTCAACGAGCTTCAGCACC | 352 | 5′-RACE for Sjgmd1 |
GMD1-5I | GGCACCGAGAGGAGCACAATCTCAGG | ||
GMD1-F | CATATGATGGCTGAGGTAATGCCCAAGGAGAGC | 963 | ORF-PCR for Sjgmd1 |
GMD1-R | GAATTCTTAGGTGGTGAGCGAGTCGGGCGA | ||
GMD2-F | CATATGATGGCCGAGCCCGAGGTGAAGAAGT | 948 | ORF-PCR for Sjgmd2 |
GMD2-R | GAATTCTTAGATGATAAGCTCGCTCGGCGAC | ||
qGMD1-F | TCCTCTCACTCTTTCGGCATCC | 128 | qRT-PCR for Sjgmd1 |
qGMD1-R | CACCCGATCTGGATGATGCTC | ||
qGMD2-F | TGCTACCTGAGCCGCAAATACG | 126 | qRT-PCR for Sjgmd2 |
qGMD2-R | CCGCCAAGAACTCCCTGAAGACC | ||
Actin-F | GACGGGTAAGGAAGAACGG | 184 | qRT-PCR for β-actin |
Actin-R | GGGACAACCAAAACAAGGGCAGGAT |