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Fig. 1 | BMC Plant Biology

Fig. 1

From: Effect of atmospheric carbon dioxide levels and nitrate fertilization on glucosinolate biosynthesis in mechanically damaged Arabidopsis plants

Fig. 1

Principal component analysis (PCA) of foliar metabolite profiles of Arabidopsis grown at different levels of CO2, nitrate fertilization and wounding stress. Plants were grown under two different atmospheric CO2 levels (ambient (440 ppm; LC) or elevated (880 ppm; HC)) and fertilized with either 1 mM or 10 mM nitrate and either not treated (control) or mechanically damaged (wound). The average of 4 independent samples were compared by 2-way analysis of variance. a Constitutive foliar metabolic profile. The purple shaded area denotes constitutive metabolites extracted from plants fertilized by 1 mM nitrate and grown at ambient CO2 compared to the red shaded area denotes constitutive metabolites extracted from plants grown at elevated CO2 levels. b Wound-induced metabolite profile. The red shaded area denotes metabolites extracted plants fertilized with 10 mM nitrate and grown under elevated atmospheric CO2. c Metabolite profile of plants grown at ambient CO2 levels (440 ppm). The blue shaded area denotes constitutive metabolites extracted from plants fertilized by 1 mM nitrate. d Metabolite profile of plants grown at elevated CO2 levels (880 ppm). Blue shaded area denotes constitutive metabolites compared to the green shaded area that denotes wound-induced metabolites extracted from plants fertilized with 1 mM nitrate. The red shaded area denotes constitutive and wound-induced metabolites extracted from plants fertilized with 10 mM nitrate. e Metabolite profile of plants fertilized with 1 mM nitrate. The purple shaded area denotes constitutive metabolites extracted from plants grown at ambient CO2 levels compared to the red shaded are that denotes constitutive metabolites extracted from plants grown at elevated atmospheric CO2. f Metabolite profile of plants fertilized with 10 mM nitrate. Green circle denotes wound-induced metabolites extracted from plants grown at ambient CO2 levels

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