Fig. 4

Expression patterns of Citrullus lanatus TCP genes in different tissues. The expression profile of ClTCP genes in the seed, leaf, internode, shoot tip, root, flowers and fruit was obtained through semi-quantitative PCR analysis. Expression of the CLYLS8 gene was monitored as an internal control. The phylogenetic tree of all TCP transcription factors in Citrullus lanatus was constructed using MEGA 5.0 with the neighbor-joining method. Support for the topology was assessed by means of a bootstrap analysis with 1000 replicates. S: Seeds at germination; C: cotyledons; L2: leaves at two-true-leaf stage; R2: roots at two-true-leaf stage; L6: leaves at six-true-leaf stage; R6: roots at six-true-leaf stage; I: internodes; SAM: shoot apical meristem; M: male flower; FM: female flower; F: immature fruit 3 hours after pollination