Fig. 6

Characterization of OsRH2 and OsRH34 double-knockdown transgenic lines. a Schematic presentation of the double silencing of OsRH2 and OsRH34 of the RNA interference construct. A 271-bp fragment at the 3′ end of OsRH2 and OsRH34 conserved region was ligated in sense and antisense orientations to the GFP cDNA and fused downstream of the Ubi promoter. b Expression of OsRH2 and OsRH34 in T1 transgenic rice seedlings. Total RNA was isolated from 14-day-old seedlings and subjected to qRT-PCR using OsRH2- and OsRH34-specific primers. Rice Act1 was used as an internal control. Error bars indicate the standard deviations (SD) of triplicate experiments. Gene expression was related to wild-type plants, as 1. * is significantly different from the wild-type plants (Student’s t test: p <0.05). OsRH2 and OsRH34 double-knockdown lines are named as RH2Ri 2b, 4, and 14b. Wild-type line is indicated by WT