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Table 8 Linkage analysis of RG-I from NbPAGR-silenced N. benthamiana plants and PAGR-overexpressing Arabidopsis lines

From: A DUF-246 family glycosyltransferase-like gene affects male fertility and the biosynthesis of pectic arabinogalactans

 

VIGS Control

NbPAGR-Silenced

Col-0

35S::PAGR-YFP line 6

35S-PAGR-YFP line 9

T-Ara

1.1 ± 0.4

1.0 ± 0.2

1.9 ± 0.5

1.4 ± 0.2

2.3 ± 0.6

2-Rha

6.2 ± 0.5

8.9 ± 0.1*

8.5 ± 1.0

7.1 ± 1.8

9.6 ± 1.1

3-Ara

0.7 ± 0.1

0.7 ± 0.1

1.3 ± 0.1

1.3 ± 0.1

1.2 ± 0.1

T-Gal

4.9 ± 0.4

6.7 ± 0.2

6.3 ± 0.3

6.0 ± 0.7

5.8 ± 0.5

5-Ara

8.4 ± 0.5

9.2 ± 0.7

16.5 ± 0.7

16.5 ± 0.6

16.0 ± 0.7

2,4-Rha

7.9 ± 0.1

8.8 ± 0.4

7.4 ± 1.2

7.6 ± 1.1

6.8 ± 0.4

3-Gal

4.4 ± 0.1

4.5 ± 0.3

3.5 ± 0.1

4.6 ± 0.2*

4.7 ± 0.2*

3,5-Ara

1.2 ± 0.1

1.4 ± 0.1*

6.4 ± 0.2

6.4 ± 0.5

5.9 ± 0.5

4-Gal

47.9 ± 0.4

42.0 ± 1.6*

19.4 ± 0.4

16.0 ± 0.6*

14.7 ± 0.6*

2,5-Ara

ND

ND

6.6 ± 0.4

6.1 ± 0.5

5.5 ± 0.5

6-Gal

2.5 ± 0.1

3.0 ± 0.2*

4.6 ± 0.3

5.6 ± 0.1*

6.3 ± 0.1*

3,4-Hex

1.7 ± 0.2

1.4 ± 0.1

8.9 ± 0.5

9.3 ± 1.3

8.0 ± 1.0

2,4-Hex

0.7 ± 0.1

0.6 ± 0.1

ND

ND

ND

4,6-Hex

1.6 ± 0.1

1.6 ± 0.2

1.8 ± 0.1

1.5 ± 0.1

1.3 ± 0.1

3,6-Hex

10.6 ± 0.2

10.4 ± 0.4

6.8 ± 0.3

10.7 ± 0.2*

11.7 ± 1.1*

  1. Values are the molar percentage of total sugars detected. Data shown are averages ± standard deviation. Asterisks indicate significant difference from wild type or control plants (p < 0.01, t-test). (ND: not detected)