Fig. 6

Functional characterization of four SOS1s in the salinity sensitive yeast mutant ANT3 (ena1 nha1) and expression analysis of SOS1 gene in four SOS1s yeast transformants. ANT3 were transformed with plasmid containing four SOS1s (+AjSOS1, +CrcSOS1, +CcSOS1, +CmSOS1), G19 (ena1) and ANT3 (ena1 nha1) were transformed with the empty vector. G19 (ena1) cells were used as a positive control. Transformants were brought to a density 2 x 10⁶ per mL, of which 5 μL (serially diluted) were spotted onto YPDA medium containing 0 mM NaCl (a) and AP medium containing 30 (b), 50 (c) and 70 (d) mM NaCl. Plates were incubated at 30 °C for 2–4 days. e qPCR analysis of SOS1 expression in four SOS1s yeast transformants. The actin gene was employed as an internal control