Fig. 8

Constitutively expressing TaBASS2 enhances ROS tolerance and reduces Na⁺ contents in wheat. a DAB staining of the leaves from two-week-old soil-grown vector control (VC) and two Ubi::TaBASS2 lines (OX1 and OX21). b The expression levels of TaCAT1 in two-week-old VC and OX seedlings. c The catalase activity in two-week-old VC and OX seedlings. d The expression levels of TaHKT1;5-D in two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using TaCyclophil in in wheat as the internal control. e, f The Na⁺ contents in the shoot and root tissue from two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the VC result (P < 0.05, t-test)