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Fig. 2 | BMC Plant Biology

Fig. 2

From: Domestication-driven Gossypium profilin 1 (GhPRF1) gene transduces early flowering phenotype in tobacco by spatial alteration of apical/floral-meristem related gene expression

Fig. 2

Profilin gene expression analysis in vegetative and reproductive tissues of two transgenic overexpression lines Pf-Ox4 and Pf-Ox17 of tobacco. a Semi-quantitative expression analysis of profilin in vegetative tissues of Pf-Ox4 and Pf-Ox17 in comparison to two independently in vitro regenerated control lines. b Semi-quantitative expression analysis of profilin in reproductive tissues of Pf-Ox4 and Pf-Ox17 in comparison to two independently in vitro regenerated control lines. c & d Average expression values of profilin gene in both vegetative and reproductive tissues of the two overexpression lines in comparison to control lines, respectively, by densitometry imaging analysis avoiding any biases visible in the band intensities on an agarose gel. e Significant increase in the plant height through elongated internodal regions of over-expression lines Pf-Ox4 and Pf-Ox17 (labeled 1 & 2) in comparison to two control lines (labeled 3&4) after 120 dpt. f In vitro regenerated control line after 100 days of vegetative growth post-transplantation. g Transgenic Pf-Ox4 line showing early conversion of apical shoot meristem into floral meristem after 100 days of vegetative growth post-transplantation. h Relative size differences in 20th leaf from the top of transgenic and control lines. i Relative size differences in 13th leaf from the top of transgenic and control lines

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