Fig. 3

Transcript expression of conserved and novel miRNA predicted from Tsuda after treatment with dark, blue light or UV-A. Relative expression (2^−ΔΔCT) of conserved BrmiR159 and predicted BrmiRC0191, BrmiRC0460, BrmiRC0323, BrmiRC0418, BrmiRC0005 was analyzed by real time PCR, and the qRT-PCR products were separated by electrophoresis. The UBQ gene was used as the internal control, and seedlings in the dark control were used as the calibrator. Lanes: M, size marker (10 bp DNA Ladder, MBI); D, dark; B, blue light; A, UV-A. Each bar indicates the mean ± SE of triplicate assays after correcting for template quantity relative to the UBQ gene. The dotted line indicates the normalized reads for the corresponding miRNAs from high-throughput sequencing