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Fig. 7 | BMC Plant Biology

Fig. 7

From: Tobacco TTG2 regulates vegetative growth and seed production via the predominant role of ARF8 in cooperation with ARF17 and ARF19

Fig. 7

Partial sequence characterization and assays of the NtARF8 protein as a transcription activator. a Partial sequence of the predicted NtARF8 protein. The NtARF8 sequence contains 843 amino acids and only the middle region is presented to indicate glutamine (Q) abundance as the characteristics of ARF transcription activators. b An EMSA assay of the NtARF8-His (ARF8-His) fusion protein, the WT probe for the auxin-responsive TGTCT element, which is present in the GH3 gene promoter, and the mutant probe. c ChIP PCR conducted with chromatins isolated from leaves of plants indicated on left, the specific YFP antibody, and primers specific to the promoter and CDS of GH3. Bands in gel indicate products of PCR conducted with DNA templates from the precipitated complex consisting of chromatin fragments and the NtARF8-YFP fusion protein (ARF8-IP), the mock precipitation in the absence of the antibody (−AB), and chromatins before immunoprecipitation (input), respectively. d GH3 expression levels quantified by RT-qPCR. The WT transcript quantity ratio of GH3 to EF1α was defined as 1 and scored accordingly for ARF8i and ARF8 +:YFP. Data shown are mean values ± SEM bars (n = 3 experimental replicates)

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