Single and multiple resistance QTL delay symptom appearance and slow down root colonization by Aphanomyces euteiches in pea near isogenic lines

Lavaud, C.; Baviere, M.; Le Roy, G.; Hervé, M. R.; Moussart, A.; Delourme, R.; Pilet-Nayel, M-L.

doi:10.1186/s12870-016-0822-4

Table 3 A. euteiches DNA amounts in roots in single-QTL NIL experiments

From: Single and multiple resistance QTL delay symptom appearance and slow down root colonization by Aphanomyces euteiches in pea near isogenic lines

Experiment (Strain)	Cross^a	Genotype	QTL^b	Day after inoculation^c
Experiment (Strain)	Cross^a	Genotype	QTL^b	2	3	4^d	5	6	7	10
1	DSP * RIL 847.50	NIL4-0b	-	NA	3,0 ± 0,5	44,9 ± 4,3	201,8 ± 21,7	387,0 ± 35,0	576,8 ± 46,8	1110,8 ± 69,2
(RB84)		NIL4-5.1b	Ae-Ps5.1	NA	2,5 ± 0,5	11,2 ± 4,3***	117,8 ± 21,7***	325,4 ± 33,5*	425,7 ± 45,7***	839,4 ± 69,2***
		NIL4-7.6a	Ae-Ps7.6	NA	2,0 ± 0,5	15,5 ± 4,3***	81,6 ± 22,6***	155,4 ± 35,0***	351,9 ± 45,3***	699,5 ± 70,6***
		90-2131	-	NA	5,3 ± 0,4	31,6 ± 4,3*	162,0 ± 21,7*	297,7 ± 34,8**	348,5 ± 45,3***	858,8 ± 69,2**
	Baccara * RIL BAP8.70	NIL7-0b	-	NA	5,3 ± 0,3	43,9 ± 4,0	294,9 ± 16,6	523,2 ± 17,1	679,7 ± 29,1	1721,5 ± 80,9
		NIL7-4.1a	Ae-Ps4.1	NA	6,0 ± 0,3	67,4 ± 4,0	260,0 ± 16,6	421,9 ± 17,1**	650,2 ± 29,1	1478,2 ± 80,9
		NIL7-7.6a	Ae-Ps7.6	NA	1,6 ± 0,6***	17,0 ± 4,0***	58,8 ± 16,6***	251,0 ± 17,1***	437,4 ± 26,1***	882,4 ± 80,9***
		PI180693	-	NA	2,0 ± 0,5***	17,3 ± 4,0***	53,7 ± 16,6***	81,6 ± 26,4***	167,5 ± 26,1***	401,7 ± 80,9***
	Baccara * RIL BAP8.195	NIL7-0b	-	NA	5,3 ± 0,8	43,9 ± 12,3	294,9 ± 22,5	523,2 ± 23,8	652,1 ± 73,9	1721,5 ± 127,2
		NIL10-1.2a	Ae-Ps1.2	NA	4,4 ± 0,8	43,3 ± 12,3	180,9 ± 22,5***	362,0 ± 23,8***	827,9 ± 67,0	1450,7 ± 127,2
		NIL10-2.2c	Ae-Ps2.2	NA	3,3 ± 0,9*	48,1 ± 12,3	229,4 ± 22,5**	407,7 ± 23,8**	527,7 ± 73,9	1360,2 ± 127,2*
		NIL10-3.1b	Ae-Ps3.1	NA	5,6 ± 0,8	47,8 ± 12,3	177,5 ± 22,5***	377,2 ± 27,6***	652,5 ± 73,9	1478,4 ± 127,2
		PI180693	-	NA	0,9 ± 1,0***	17,3 ± 12,3***	53,7 ± 22,5***	88,1 ± 33,8***	167,5 ± 67,0***	401,7 ± 127,2***
	Baccara * 552	NIL7-0b	-	NA	5,3 ± 0,6	43,9 ± 7,0	294,9 ± 19,6	523,2 ± 21,4	694,8 ± 36,6	1721,5 ± 116,2
		NIL13-2.2a	Ae-Ps2.2	NA	6,0 ± 0,6	77,5 ± 7,0	186,8 ± 21,5***	344,9 ± 21,4***	549,2 ± 32,9**	1117,6 ± 143,0**
		NIL13-3.1a	Ae-Ps3.1	NA	5,1 ± 0,6	43,7 ± 7,0	168,4 ± 19,6***	247,7 ± 21,4***	451,5 ± 32,9***	991,5 ± 116,2***
		NIL13-7.6b	Ae-Ps7.6	NA	1,7 ± 0,6***	19,9 ± 7,0*	76,9 ± 19,6***	178,9 ± 29,7***	341,5 ± 38,1***	681,7 ± 116,2***
		552	-	NA	NA	4,2 ± 8,3***	14,0 ± 19,6***	82,8 ± 21,4***	177,0 ± 32,9***	428,6 ± 116,2***
2	Puget * RIL 831 .08	NIL1-0b	-	NA	2,6 ± 0,4	32,6 ± 7,7	91,8 ± 26,3	293,5 ± 7,9	535,0 ± 35,0	1349,9 ± 158,7
(Ae109)		NIL1-4.5b	Ae-Ps4.5	NA	NA	NA	NA	7,3 ± 7,6***	27,8 ± 35,0***	88,2 ± 158,7***
		RIL 831.08	-	NA	NA	NA	30,6 ± 27,1**	26,4 ± 9,1***	92,1 ± 39,6***	304,5 ± 166,6***

^a“Recipient x donor” cross lines from which each NIL was produced in the previous MAB scheme [25]. ^bQTL introgressed in each NIL from the previous MAB scheme [25]. ^cPathogen DNA amount were obtained on each genotype and scoring day from the LMM analysis of each set of lines in experiments #1 and #2. LSMeans and standard errors of pathogen DNA amount are presented in thousand DNA copies (10^{^3}). In each set of lines, significant differences of LSMeans DNA amount between the single-QTL NILs or the resistant control, and the control- NIL without QTL are indicated by *(0.01 < P < 0.05), **(0.001 < P < 0.01) and ***(P < 0.001). ^dIn experiments #1 and #2, LSMeans DNA amount were estimated from data obtained in one biological replicate at the fourth day. NA: Not Available data due to copy number <10³

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