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Table 4 Correlation parameters revealed among different classes of carotenoid types and abundance of transcripts coding for six major enzymes based on qRT_PCR

From: Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root

Total acyclic carotenoid correlation

Enzyme

cDNA code in microarray data base

Cassava genome code

p value

   ZDS

CV03051B1A05.f1

cassava4.1_004265m

0.109

Total β-carotene correlation

   LCYe

CV03088A2B02.f1

cassava4.1_005406m

0.067

   NCED3

CV03105A1F08.f1

cassava4.1_029535m

0.046

   PDS

CV03049A2F07.f1

cassava4.1_004359m

0.037

   PDS

CV03110A2H07.f1

 

0.139

   ZEP

CV03090B2B05.f1

 

0.003

   ZEP

CV03104A2A10.f1

 

0.009

Total Xanthophyll correlation

   LCYe

CV03127A2F10.f1

 

0.007

  1. Carotenoid intermediates were pooled based on classes of carotenoid end products including cyclic (β-carotene + β-xanthophylls), and acyclic (phytoene + phytofluene + ζ-carotene + neurosporene + β-zeacarotene + lycopene) carotenoids. Pearson product moment correlation coefficient (r) was obtained for linear association of total cyclic, total acyclic, total β − carotene and total xanthophylls against abundance of transcripts coding for enzymes related to carotenoids synthesis. The values of SE of r accounts for the standard error of the estimates by the linear regression and represents the level of accuracy of predictions. If probability that r = 0, P(r = 0) is < =0.05, r is significantly different from 0 and some degree of correlation is shown. The value n refers to number of replication