Table 1 Identification of differentially expressed proteins spots from 2-DE gels

1.1.1.2 C1 Metabolism

 21

RuBisCO large subunit-binding

410

11 %

Egrandis_v1_0.005399 m

6

0.4

 58

Ribulose bisphosphate carboxylase large chain

325

15 %

RBL_ANTFO

6

0.54

 59

RuBisCO large subunit-binding

263

13 %

Egrandis_v1_0.005399 m

4

0.74

 73

Formate dehydrogenase

1297

39 %

Egrandis_v1_0.015998 m

11

1.93

 7

Phosphoglycerate kinase

3928

58 %

Egrandis_v1_0.014782 m

16

0.48

1.1.2 Energy Metabolism (Carbon)

 8

Enolase

122

5 %

Egrandis_v1_0.021648 m

1

0.46

 14

Phosphoglycerate kinase

1681

46 %

Egrandis_v1_0.014782 m

12

0.3

 51

Phosphoglycerate kinase

1452

32 %

Egrandis_v1_0.014782 m

8

0.4

 52

Enolase

128

3 %

Egrandis_v1_0.010202 m

5

3.77

 60

Triosephosphate isomerase

1092

45 %

Egrandis_v1_0.020110 m

9

0.56

 101

Enolase

1610

19 %

ENO1_HEVBR

6

1.33

 114

Enolase

929

15 %

ENO1_HEVBR

4

0.43

1.1.3 Energy Transfer/Atp-Proton Motive Force

 3

HSP20-like chaperones superfamily protein

153

28 %

Egrandis_v1_0.029820 m

2

3.98

 25

ATPase, V1 complex, subunit B

964

27 %

Egrandis_v1_0.010528 m

10

0.35

 30

ATP synthase alpha/beta family protein

1664

51 %

Egrandis_v1_0.007569 m

17

0.36

 46

Citrate synthase

286

21 %

Egrandis_v1_0.011280 m

7

1.85

 91

Citrate synthase

523

18 %

Egrandis_v1_0.011280 m

5

1.56

 97

Malate dehydrogenase

1094

30 %

Egrandis_v1_0.018951 m

6

0.62

1.2.1 Amino AcidMetabolism

 71

Phosphoglycerate dehydrogenase

456

35 %

Egrandis_v1_0.014972 m

7

0.54

1.2.3 Nucleotide/Nucleoside and Nucleotide Sugar Metabolism

 2

UDP-sugar pyrophosphorylase

395

10 %

Egrandis_v1_0.005961 m

3

0.48

 41

Adenosinekinase

1965

45 %

Egrandis_v1_0.018389 m

10

1.96

 61

UDP-glucose pyrophosphorylase

89

6 %

Egrandis_v1_0.011888 m

2

2.08

 63

UDP-glucose dehydrogenase

554

22 %

Egrandis_v1_0.010940 m

6

1.9

 103

UDP-glucose pyrophosphorylase

621

32 %

Egrandis_v1_0.011888 m

7

0.49

 106

UDP-glucose pyrophosphorylase

1187

41 %

Egrandis_v1_0.011888 m

13

0.46

1.2.8 Secondary Metabolism

 87

NAD(P)-linked oxidoreductase

445

24 %

Egrandis_v1_0.019083 m

5

2.05

 96

Phenylcoumaranbenzylicether reductase

2400

76 %

Egrandis_v1_0.020543 m

15

0.45

2.1 Cell Processes

 78

Vacuolar H + -ATPasecatalyticsubunit

933

20 %

Egrandis_v1_0.006156 m

8

0.41

 70

14-3-3 protein

654

26 %

Egrandis_v1_0.043757 m

6

0.45

 123

14-3-3- protein

1031

39 %

Egrandis_v1_0.023614 m

7

0.49

2.2.2 Protection Responses/Detoxification

 1

Ascorbate peroxidase

1788

41 %

Egrandis_v1_0.024254 m

6

4.71

 24

Peroxidase

209

9 %

gi|242089639

2

1.9

 31

Ascorbate peroxidase

84

7 %

Egrandis_v1_0.024254 m

1

0.64

 33

Ascorbate peroxidase

1406

41 %

Egrandis_v1_0.024164 m

6

0.49

 45

Ascorbateperoxidase

121

10 %

Egrandis_v1_0.024164 m

1

1.93

 47

Glutathioneperoxidase

75

8 %

Egrandis_v1_0.024172 m

2

4.22

 69

Glutathione S-transferase, C-terminal-like

157

8 %

Egrandis_v1_0.022631 m

2

0.52

 74

Ascorbateperoxidase

1624

57 %

Egrandis_v1_0.024217 m

6

4.11

 79

Ascorbateperoxidase

1447

51 %

Egrandis_v1_0.024254 m

7

3.28

 99

Ascorbateperoxidase

200

14 %

Egrandis_v1_0.024254 m

2

2.32

 107

Copper/zinc-superoxidedismutase

296

19 %

Egrandis_v1_0.029096 m

2

1.42

2.2.3.2 Abiotic

 90

Late embryogenesisabundantprotein

1384

43 %

Egrandis_v1_0.020038 m

13

0.55

4.1.2.4 LigninMetabolism

 105

Caffeicacid 3-O-methyltransferase

383

31 %

COMT1_EUCGU

7

0.49

 124

Caffeicacid 3-O-methyltransferase

328

3 %

Egrandis_v1_0.003388 m

6

0.57

4.1.2.5 Expansins, Xetand Extensin

 54

Major Latex protein MLP-like

1055

50 %

Egrandis_v1_0.029781 m

6

0.71

 95

Major Latex protein (MLP-like)

792

55 %

Egrandis_v1_0.029781 m

6

0.83

4.3 Cytoskeleton

 17

Beta-tubulin

267

13 %

Egrandis_v1_0.012369 m

4

0.42

 102

Beta-tubulin

1533

27 %

Egrandis_v1_0.012451 m

11

0.46

 111

Beta-tubulin

2034

34 %

Egrandis_v1_0.012369 m

15

0.79

5.2 Rna Related

 44

Glycine-rich RNA-binding protein

283

21 %

Egrandis_v1_0.020038 m

4

0.36

5.3.3 Translation Related

 66

Eukaryoticinitiationfactor

381

9 %

Egrandis_v1_0.014208 m

3

2.56

 75

Elongationfactor 1 beta

684

14 %

Egrandis_v1_0.021389 m

3

0.34

 84

Eukaryoticinitiationfactor

1471

41 %

Egrandis_v1_0.014208 m

13

1.83

5.3.5 Protein Folding/Chaperoning

 4

Heatshockproteins

565

28 %

Egrandis_v1_0.021389 m

5

1.58

 11

Heatshockprotein

2052

28 %

Egrandis_v1_0.044829 m

15

2.53

 13

Heatshockprotein

230

13 %

Egrandis_v1_0.027871 m

2

0.32

 40

Heat shock cognate 70 kDa protein

1888

31 %

Egrandis_v1_0.003388 m

18

2.72

 57

Heatshockproteinmitochondrial

385

21 %

Egrandis_v1_0.026204 m

3

1.89

 62

Heatshockprotein (HSP20)

1331

50 %

Egrandis_v1_0.045806 m

9

2.99

 67

Heatshockprotein

157

15 %

Egrandis_v1_0.029182 m

2

4.11

 76

Heatshockprotein (Hsp20)

1589

26 %

Egrandis_v1_0.021389 m

7

1.52

 82

Heatshock 70 kDaprotein

3155

44 %

Egrandis_v1_0.005502 m

23

3.84

 94

Heatshockprotein

810

34 %

Egrandis_v1_0.029182 m

5

1.54

 98

Heatshockprotein (HSP20)

363

23 %

Egrandis_v1_0.026663 m

4

1.8

 104

Heatshockprotein

383

18 %

Egrandis_v1_0.026663 m

5

1.99

 112

Heatshockprotein

2682

47 %

Egrandis_v1_0.025526 m

9

0.53

 118

Heatshockcognate 70 kDa

1292

24 %

Egrandis_v1_0.003884 m

14

0.53

 120

Heatshockprotein

1010

22 %

Egrandis_v1_0.044829 m

11

1.84

 125

Heat shock 70 kDa protein, mitochondrial

1413

29 %

Egrandis_v1_0.004853 m

13

1.66

5.3.6 Protein Cleavage and Turnover

 42

Proteasomalregulatoryprotein

187

14 %

Egrandis_v1_0.018412 m

4

1.97

 55

Proteasomesubunit beta type

1215

34 %

Egrandis_v1_0.021324 m

7

1.78

 68

26S proteasome non-ATPase regulatory particle

533

28 %

Egrandis_v1_0.023175 m

4

0.63

6.2 Putative Protein

 116

NAD(P)-binding Rossmann-fold superfamily protein

386

21 %

Egrandis_v1_0.018330 m

6

0.72