Fig. 4

Increased levels of anthocyanin accumulation and acid phosphatases activity in bik1 plants. a and b Measurements of anthocyanin contents. Seven-day-old WT and bik1 seedlings grown on 1/2 MS were transferred into MS medium supplemented with Pi (+Pi, 1 mM) or without Pi (−Pi). Photos were taken and anthocyanin contents were measured at 5 days after treatment. c and d Detection of acid phosphatase activity. Ten-day-old seedlings grown in liquid medium were transferred to fresh medium supplemented with Pi (+Pi, 1 mM) or without Pi (−Pi) and covered with a layer of 0.008 % BCIP-containing agarose. Photos were taken and the activity of acid phosphatase in roots was estimated at 5 days after treatment. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level between WT and bik1 plants grwon under same condition