Fig. 6

Down-regulated expression of Pi starvation-induced genes in bik1 plants. a Expression patterns of Pi starvation-induced genes in WT and bik1 seedlings grown under + Pi and –Pi conditions. Seven-day-old seedlings grown under normal Pi condition were treated with Pi (+Pi, 250 μM) or without Pi (−Pi) for 5 days and total RNA were extracted from root tissues at 5 days after treatment. b Expression patterns of PHO2 and miRNA399 in WT and bik1 seedlings grown under + Pi and –Pi conditions. Seven-day-old seedlings grown under normal Pi condition were transferred to medium supplemented with Pi (+Pi, 250 μM) or without Pi (−Pi) and samples were collected at 48 h after treatment. Expression of Pi starvation-responsive genes and miR399s was analyzed by qRT-PCR using specific primers and data were normalized with the transcript level of UBQ10 as an internal control. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level