Fig. 7

Overexpression of soyBiPD in Arabidopsis attenuates the PEG and tuncamycin–mediated induction of DCD/NRP–mediated cell death signaling genes. a and b AtNRP1, AtNRP2 and ANAC036 expression in Col–0 and BiPD–overexpressing lines. Total RNA was isolated from 15 days–old Arabidopsis plants treated with PEG (10 % w/v) and Tunicamycin (2,5 μg/mL) for 2 h, 6 h, 12 h and 24 h. H2O was used as control for PEG and DMSO for Tunicamycin. The transcript levels of selected genes were quantified by qRT–PCR. Gene expression was calculated using the 2^-ΔΔCt method and UBQ5 as endogenous control. cDNAs were obtained from four biological replicates and validated individually. (S.E., n = 4 biological replicates). BiPDox T07 and BiPDox T23 are transgenic Arabidopsis lines independently transformed with the soyBiPD gene. a, c, e, g Time course of PEG-induced RAB18, AtNRP1, AtNRP2 and ANC036 in Col-0 and BiP-overexpressing lines. b, d, f, h Time course of tunicamycin-induced CNX, AtNRP1, AtNRP2 and ANC036 in Col-0 and BiP overexpressing lines