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Fig. 6 | BMC Plant Biology

Fig. 6

From: The sucrose non-fermenting 1-related kinase 2 gene SAPK9 improves drought tolerance and grain yield in rice by modulating cellular osmotic potential, stomatal closure and stress-responsive gene expression

Fig. 6

Monitoring stomatal closure and relative expression level of stomatal genes in SAPK9 overexpressed (OE) and gene silenced (RNAi) transgenic rice plants. a-i Representative photographs showing stomatal status in rice leaves. a-ii Number of completely open, partially open and completely closed stomata were calculated before (Normal) and after stress (Drought) in three sets of plants, i.e. OE, RNAi and NT plants (n = 80). All the results were compiled from three independent experimental sets. Error bars represent the mean ± SD of triplicate measurements. Student’s t-test was performed to find out statistically significant differences (**P < 0.01). b Real-time PCR analysis showing transcript level of stomatal genes- (i) OsSLAC1 and (ii) OsSLAC7 in transgenic and NT plants during drought stress. For internal reference, the OsUbi1 gene was used. Error bars represent the mean ± SD of triplicate measurements. Student’s t-test was performed to find out statistically significant differences (**P < 0.01)

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BMC Plant Biology

ISSN: 1471-2229

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