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Fig. 8 | BMC Plant Biology

Fig. 8

From: The sucrose non-fermenting 1-related kinase 2 gene SAPK9 improves drought tolerance and grain yield in rice by modulating cellular osmotic potential, stomatal closure and stress-responsive gene expression

Fig. 8

Expression profiling of a few drought stress inducible genes working hierarchically downstream of the ABA-signaling pathway and analysis of subcellular localization of SAPK9 protein in onion epidermal cells. a-i-vi Analysis of real-time PCR depicting transcript level of TRAB1, OsbZIP23, OsbZIP46, OsLEA3-1, OsRab16B and OsRab21 in leaf tissues of transgenic and NT plants during drought stress. For internal reference, the OsUbi1 gene was used. Error bars represent the mean ± SD of triplicate measurements. Student’s t-test was performed which indicated statistically significant differences (**P < 0.01). b SAPK9–GFP fusion protein and control GFP were transiently expressed in onion epidermal cells and observed with a laser-scanning confocal microscope. Upper panel (i-v): SAPK9–GFP fusion; lower panel (vi-x): only GFP. Images were taken (i, vi) in the dark field for green fluorescence, (ii, vii) in the bright field, (iii, viii) dark field green fluorescence merged with bright field image and (iv, ix) DAPI stained nucleus (5 μg/mL) (v, x) DAPI stained nucleus merged with dark field green fluorescence. Scale bar- 100 μm

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