Table 3 Markers used in the study
Name | Chromosome | Position | Forward primer | Reverse primer | Enzyme | Resistant allele fragment size | Susceptible allele fragment size | Reference |
|---|---|---|---|---|---|---|---|---|
dCAPS2 | 3 | 3:10,431,528 | CCTCCTCTGTTGGGAAATCA | TCTGAAGGCCTGTGTTAAGCT | Hind III | 307 | 286 21 | this study |
dCAPS3 | 4 | 4:15,255,162 | AGTACGGCCTCAAACAGTGG | GAAAATTACAATCAAATGGAGCT | SacI | 303 | 283 20 | this study |
CAPS1 | 5 | 5:5,178,332 | ACTTCACTGGGTGGACTTGC | ATTCTCAGGCATGGTCAAGG | TaqI | 89 364 | 453 | this study |
CAPS2 | 5 | 5: 5,179,402 | AGGTGAAATTGGTTGGAAGG | CCCATGTCAGAAGCATCATC | Hpy 188III | 104 129 43 14 37 | 104 172 14 37 | this study |
dCAPS1 | 5 | 5,454,538 | AGCTGTGGAATGACGACTAG | TTACAACACCCAGTGCGTTC | SPE1 | 324 | 304 20 | this study |
CAPS3 | 5 | 5: 5,622,070 | TGCTCAGCTGCTATACCAAGA | CACAATGCCTGATGGAGAGA | XcmI | 46 398 | 444 | this study |
CAPS4 | 5 | 5: 5,662,479 | GAACCAGTTCAAGCGACTCC | CGAACTTAGAGGCCAAAACG | BanI | 108 193 | 301 | this study |
CAPS6 | 5 | 5:5,730,691 | CTGAATGGGTTTATGCGTTG | ATCAATGGCCCCTCTCTTTT | PsiI | 306 | 139 167 | this study |
CAPS12 | 5 | 5:5,953,917 | TGCATGTCAACGAAAACTCA | GTAGAGGGGGTTTTCCGAAG | TaqI or HinfI | 188 (190) 122 (120) | 310 | this study |
CAPS13 | 5 | 5:5,974,663 | CGCAGCGAATGTTATCACTG | TTGCTGTGAAATTGCAGCTC | TaqI | 221 180 | 401 | this study |
CAPS8 | 5 | 5:6,992,170 | CGCCCTCCGTGTATTCTAAA | GGCTGCTTCACTTACCAAGC | BstYI | 419 | 330 89 | this study |
CAPS14 | 5 | 5:6,066,948 | CCGAGCATTGAGGTTGGTAT | CTAAGGCGAGCTGCTGAAGT | NheI | 322 | 165 157 | this study |
CAPS9 | 5 | 5:7,212,649 | TGGCATGAAATGAGTTAAAAGTG | TCCTGAACTTGGGGTTATGG | RsaI | 66 366 | 66 173 193 | this study |
CAPS11 | 5 | 5:7,551,254 | ACAAGCTGATGGGCAAACTC | GACGGATCCGAGTGTTTGTT | AseI | 100 238 | 100 113 125 | this study |
TETRA1 | 1 | 26,370,595 | O:CCGAAGATGTGTGATTCATG I:GACGATACTTGTCCAGATAT | O:GAAGGGATTTTGTTAGGAGT I:AGCACTCAAGATGAAAGTGATC | - | Â | Â | this study |
TETRA2 | 2 | 23,741,639 | O:ACTATCTGACCGAAAGGAA I:TTGGTACCAAATTCTGCACT | O:ATCTGCTGACAGGAGAATTCA I:ATCTTACGGTGAAGGACATT | - | Â | Â | this study |
TETRA3 | 7 | 13,713,780 | O:TAGCTGGTCCGTGTACTTTA I:TTTCCATTGTGGGTCGTGGAGT | O:GGAACTATGCTTTGGGACTT I:ATTCTTGTAGCATCATCAAAACT | - | Â | Â | this study |
TETRA4 | 10 | 3,159,416 | O:ATACTGGAGGGTTGTTTCTA I:TAAGCGTGCGCAGCCATAAACAAG | O:CGGTCTCAGAATCATAGTCTTG I:GGGTTTTTTCGGAAATTCAAAG | - | Â | Â | this study |
DMB-SSR-158 | 5 | 5:5,597,891 - 5:5,590,645a | TGGAAAATTTGCAGCAGTTG | ATTGATGGAGGGCGGAAGTA | Â | 300-400 | ||
779 | 5 | 7351532-7351612 | CTAATAAATCATCTATACgTCTCTC | ATTgCTATTTAgCgAATAATAgTAC | Â | Â | [13] | |
OPW 02a4 | 5 | 6743545 – 6745026a | CCAAAggAgTCgAgTgAAACT | CAACAACCCTTCCTCTATCTC |  | 400 – 1,100 | [13] | |
Vr34480 | ? | ? | AATTCTTgATTggTCCACATg | AAAAAATTACACCTCgTTCg | Â | 500 | [13] | |
MB-87 | ? | Fwrd:3:11,421,121 | TCCCTTGTGGGAGATCCT | CTTTGCCACACTCCTTGC | Â | ~300 | [21] | |
4:15,255,162 | ||||||||
5:5,602,099 | ||||||||
Rev: −b | ||||||||