Fig. 4

Malate accumulation following the onset of ripening in transgressively cool conditions. Each point represents one cluster (~30 berries). At the end of each treatment, all clusters were harvested simultaneously on 5 plants. The induction of ripening is marked by the simultaneous inductions of malate breakdown and massive hexose storage in berries at 30–20 °C, as typically observed in vineyard conditions. The onset of malate breakdown is delayed to 0.4–0.5 M hexoses following a 3 months growth period at 22–12 °C