Fig. 3

SCAP1 controls GCs development. (a) Number of Guard cells (GC), pavement cells (PC) and stomatal index in wild type (Ler), and scap1-2 mutants and, (b), wild type (Col) and T3 homozygous pro35S:amiRNA2-SCAP1 (amiRNA2-SCAP1, line #2). (c) Morphological alterations observed in pro35S:SCAP1-YFP (35S:SCAP1) lines at different developmental stages (seedlings, rosette, bolting plants). (d) Number of guard cells (GC), pavement cells (PC) and stomatal index in wild type (Col) and a T3 homozygous pro35S:SCAP1-YFP (35S:SCAP1) intermediate line (line #7). (e) GUS staining of double proAtMYB60:GUS pro35S:SCAP1-YFP (35S:SCAP1) or single proAtMYB60:GUS (WT Col) hemizygous lines. Shown are mature cotyledons (inset, higher magnification of a representative cotyledon area) and the first leaf of 10 days old seedlings. Bar = 200 μm (inset, 25 μm). (f) Representative abaxial epidermal phenotype of the 6^th expanded leaf of wild type (Col), pro35S:amiRNA2-SCAP1 (amiRNA2-SCAP1, line #2) and pro35S:SCAP1-YFP (35S:SCAP1, line #7) mutants. Guard cells are false coloured in black. Bar = 50 μm. In a, b, d, (**), (^XX) and (°°) = P < 0.01 (two tails T Student test) for comparisons between the wild type and the mutant alleles for GC, PC cell density or stomatal index, respectively. ns = not significant. Error bars = Standard Error