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Fig. 4 | BMC Plant Biology

Fig. 4

From: A novel role for STOMATAL CARPENTER 1 in stomata patterning

Fig. 4

SCAP1 affect stomata spacing and induce meristemoid production. (a-d) Morphological alterations observed in 4-weeks old wild type (Col) or (e-h) pro35S:SCAP1-GR (35S:SCAP1-GR) plants grown in presence of DEX (c-d and g-h) or mock (a-b and e-f). (f, h) GUS staining of double proAtMYB60:GUS pro35S:SCAP1-GR or (B, D) single proAtMYB60:GUS hemizygous plants. (i-j) Confocal images of MUTE-YFP fusion proteins in hemizygous proMUTE:MUTE-YFP or (k-l and n) double hemizygous proMUTE:MUTE-YFP pro35S:SCAP1-GR transgenic plants. Insets (j and l) are higher magnification of the areas shown in (i) and (k), respectively. White arrowhead in (n) indicates two adjacent meristemoid. (m) Number of epidermal cells accumulating MUTE-YFP protein in hemizygous proMUTE:MUTE-YFP or double hemizygous proMUTE:MUTE-YFP pro35S:SCAP1-GR plants at different developmental stages (5 and 10 das). Shown is the average number of observable nuclei expressing MUTE-YFP in epidermal cells of 10 independent 1st leaf primordia. Note that at stage 5 das, numbers refer to the entire primordium while at 10 das numbers refer to an area of 562 mm2. Error bars = Standard Error. ** = P < 0.01 two tails T Student test. Bars = 1 mm (a, c, e, g,); 200 μm (i, l); 100 μm (j, m); 50 μm (b, d, f); 20 μm (h, o)

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