Fig. 3From: BnPME is progressively induced after microspore reprogramming to embryogenesis, correlating with pectin de-esterification and cell differentiation in Brassica napus Expression analysis of BnPME gene by quantitative RT-PCR during stress-induced microspore embryogenesis. Histogram expresses relative changes of expression at different developmental stages: vacuolated microspore (starting point, before reprogramming induction), early proembryo, and globular embryo. Each column represents the mean of two independent reactions with three replicates of each reaction. Transcript levels were normalized to vacuolated microspore levels. Bars indicate the SEM. Different letters on columns indicate significant differences at P < 0.05Back to article page