Fig. 5

Constitutive expression of VSSs does not strongly perturb the miR159 silencing of MYB33/65. a Different phenotypes developed in 28-day-old 35S-P19 and 35S-P0 primary transformants with wild-type (Col) grown alongside as a control. b The representative classification of developmental defects among 35S-P0 primary transformants. Class I: wild-type-looking; Class II: mild reduction in rosette size and partially curled leaves; Class III: all leaves curled group; Class IV: severely stunted and all leaves curled. c qRT-PCR analysis of relative mRNA levels in the different classes. Significant difference in values from the control is indicated with an _*. d Comparison of P0 mRNA levels between 35S-P0(Col) and 35S-P0(myb33.myb65) with the same classified phenotypes. The RNA samples were extracted from 26-day-old plants. Col and myb33.myb65 were used as controls. P0 mRNA levels were normalized to UBIQUITIN (At4g05320), while MYB33/65 and CP1 were normalized to that of CYCLOPHILIN. Values are the mean of three technical replicates with error bars representing the SD. Significant differences between 35S-P0(Col) and 35S-P0(myb33,65) values are indicated with an _*