Fig. 1

Domain arrangement of PDI8. a The secondary structure of PDI8. Positions of α-helices (E) and β-strands (H) are based on prediction by SPIDER2. The thioredoxin-fold domains (a, b and b’), and predicted signal peptide (SP) and TMD of PDI8 are boxed. Each thioredoxin fold consists of 5 β-strands and 4 α-helices (underlined), in the arrangement β₁-α₁-β₂-α₂-β₃-α₃-β₄-β₅-α₄. b Comparison of the domain organizations of Homo sapiens TMX3 and Arabidopsis PDI8, showing the relative positions of the SP, TMD, and domains a, b and b’. PDI8^abb’ and PDI8^bb’ represent truncated forms of PDI8 used in this study