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Fig. 6 | BMC Plant Biology

Fig. 6

From: Molecular cloning and characterization of a flavonoid-O-methyltransferase with broad substrate specificity and regioselectivity from Citrus depressa

Fig. 6

Bioproduction of polymethylated quercetin using recombinant E. coli carrying CdFOMT5. a The HPLC analysis of the reaction mixture identified five peaks: (1) 3-O-methylated quercetin; (2) isorhamnetin (3′-O-methylated quercetin); (3) azaleatin/tamarixetin; (4) dimethylated quercetin; (5 and 6) trimethylated quercetin; and (7) 3,5,7,3′-tetramethylated quercetin. Peaks labeled with ‘x’ were not identified as quercetin derivatives. Transformant cells harboring empty vector (pET21b) was used as host cell. b Mass spectrometry analysis of purified peak 7 product. c NMR spectrum of purified product (peak 7). d Predicted structure of 3,3′,5,7-O-methylated quercetin

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