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Fig. 4 | BMC Plant Biology

Fig. 4

From: A fast and simple LC-MS-based characterization of the flavonoid biosynthesis pathway for few seed(ling)s

Fig. 4

Reduction of seed material and proof of principle with seeds. Reduction of seed material down to one seed (a,b) and application to a set of selected mutants (c-e) with extracted non-hydrolysed, extracted hydrolysed and non-extracted hydrolysed samples analysed by LC-ESI-MS-QTRAP(MRM). a Flavonoids with detectable and quantifiable responses in 50, 25, 10, 5 and 1 A. thaliana Col-0 seed(s). Grey: LOD but not LOQ is passed, black: LOQ is passed for the majority of replicates. See also Additional file 6: Figure S9 and Additional file 5: Figure S7 and S8 (effect of hydrolysis on analysed flavonoids). b Responses normalized with the respective deuterated internal standard. Several scales are used for each subfigure. Grey values of scales correspond to the grey values of the substances. Boxed: optimal seed number according to this experiment. Error bars = STDEV. (Additional file 6: Table S11: statistics) c-e) Mutant analysis: Heatmaps showing log2 of fold differences relative to the respective wild types. d Same as in (c) and (e) for tt7-1 with appropriate scales. e Same data as in (c) but normalized with kaempferol. -: LOD not passed, #: LOD but not LOQ passed, +: LOQ passed for the mutant but not for the wild type. Lines in grey shades group the three sets of mutants: enzymes, TTG1-MBW complex components, light signalling mutants. Additional file 7: Tables S12 and S13: statistics. Additional file 7: Figure S10: responses normalized with respective deuterated internal standard. c Detected catechin which is most likely derived from epimerization of epicatechin (see Fig. 3)

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