Fig. 1

a Western blot analysis to detect HA-tagged proteins in plant lines subjected to microarray analyses.35S:ROXY19 _SSMS line #18 was only used later for transcript analysis by qRT-PCR. b Principal component analysis of the normalized transcriptome data. Symbols: O, ∆, +, X and ◊, represent biological replicates of WT/Col-0, 35S:GRXC2, 35S:ROXY19 _SSMS line #9, 35S:ROXY19 l ine #8 and 35S:ROXY19 line #12, respectively. c Clustering of 1486 differentially expressed in at least of the four transgenic lines. Genes were clustered into 10 prototypes according to their normalized expression pattern using the MarVis software (upper panel). The width of each prototype column is proportional to the number of genes assigned to this prototype. The lower panel shows the normalized expression profiles of the individual transcripts. The program color codes the relative expression of a given prototype (upper panel) or transcript (lower panel) in the five genotypes. Red depicts the highest relative expression, blue the lowest (see color scale)